Journal: Translational Vision Science & Technology
Article Title: Aqueous Humor Exosomal Membrane Proteins: Decoding Pathogenic Molecular Signatures in Retinitis Pigmentosa
doi: 10.1167/tvst.14.12.15
Figure Lengend Snippet: EVArray-identified AH exosomal membrane proteins from RPs and controls. ( a ) PLS-DA plot of 21 samples (absolute expression values). ( b ) PLS-DA plot of 21 samples (relative expression values). ( c ) Volcano plot of absolute quantification data. ( d ) Volcano plot of the relative quantification metrics. Red and blue dots represent proteins with an absolute value of log 2 (FC) ≥0.26 and adjusted P < 0.05; gray dots indicate proteins that failed to pass these conditions. ( e ) Boxplots of upregulated and downregulated AH exosomal membrane protein expression (relative quantification). O range indicates RP, and blue indicates control. FC, fold change; RFU, relative fluorescence units. * P < 0.05, ** P < 0.01, **** P < 0.0001.
Article Snippet: Following three sequential wash cycles with PBS (200 μL per wash), detection was initiated by the addition of 150 μL biotinylated exosomal marker membrane protein antibody (LifeSpan BioSciences, Seattle, WA, USA; 1:1000–1:1500 dilution range) with 60-minute incubation at 25°C.
Techniques: Membrane, Expressing, Quantitative Proteomics, Control, Fluorescence